GHRP-2 & GHRP-6: Injectable Growth Hormone Releasing Hexapeptides

Getting straight to the point, GHRP signals the pituitary to secrete GH. This action offers a lot of benefits, which will be covered later. It's also worth mentioning that there are various types of GHRPs, however, this particular discussion revolves around GHRP-2 and GHRP-6.

How do GHRP-2 and GHRP-6 differ from Each Other?
While both of these amino acid peptides release GH, there's a noticeable difference in GHRP-6, which speeds up digestion allowing for larger consumption of food. It's worth adding that this particular peptide is a first-generation GHRP.

What distinguishes GHRP-2 is its ability to cause growth hormone to be released more intensely. This second-generation GHRP is best for those who want to get the absolute most value from the GH releases. On the other hand, if increasing low appetite is the main concern GHRP-6 would be best.

What Other Benefits do GHRP-2 and GHRP-6 provide?
Just one dose of GHRP can battle aging effects, improve sleep quality and repair small injuries. So in short, taking these amino acid peptides will greatly improve life and overall health.

The best time to administer a dose is right before bed since this is the period when the pituitary is most active. Some researchers administer multiple doses in a day because this can help build lean muscle tissue and promote fat loss.

If the Goal of this research peptide is fat loss, how should GHRP be used?
ghrp_6 One possible way is to use GHRP an hour before cardio, when the stomach is empty. Afterward, one isn't to eat for another two hours so that the body uses fat stores as fuel. But keep in mind that this method of promoting fat loss with GHRP is debatable.

Why not just use Growth Hormone Instead?
For starters, not all GH is pure. Many researchers have been burned when they spend a fortune to order GH from China and other countries, thinking all along that they're getting the real thing. But what they get instead is a counterfeit kit that only contains a fractional amount of GH or just HCG powder.

Growth Hormone Releasing Hormone CJC-1295

It increases protein synthesis and stimulates the growth of new muscle tissue.

- Allows for normal growth in short children with GH deficiency.
- Increases muscle mass (and physical strength if combined with moderate exercise).
- Reduces wrinkling of the skin and some other effects of skin aging.
- Re-grows internal organs that have atrophied with age.
- Causes hyperplasia, the increase of more muscle cells.
- It increases muscle mass through the creation of new muscle cells (which differs from hypertrophy).
- It promotes lipolysis, which results in the reduction of adipose tissue (body fat).
- Increased bone density.
- Faster recovery from exercise, exertion, and injuries.
- Strengthen the immune system.

It is important to begin the discussion of CJC-1295 with a discussion of the parent of the Growth Hormone Releasing Factors which is somatocrinin., this peptide ultimately gave birth to the newer generations of Growth-hormone-releasing hormone peptides (CJC-1295).

Growth-hormone-releasing hormone (GHRH), also known as growth-hormone-releasing factor (GRF or GHRF) or somatocrinin, is a 44-amino acid peptide hormone produced in the arcuate nucleus of the hypothalamus. GHRH is released from neurosecretory nerve terminals of these arcuate neurons, and is carried by the hypothalamo-hypophysial portal circulation to the anterior pituitary gland where it stimulates growth hormone secretion. GHRH stimulates the production of growth hormone.

GHRH gave birth to a more compact growth hormone releasing factor known as Sermorelin which is a synthetic analogue of growth hormone releasing hormone, which is produced by the hypothalamus. Sermorelin acetate is the acetate salt of an amidated synthetic 29-amino acid peptide that corresponds to the amino-terminal segment of the naturally occurring human growth hormone-releasing hormone (GHRH or GRF) consisting of 44 amino acid residues.

So, what was the problem with Sermorelin, or GHRH for that matter? The clinical use of growth hormone-releasing hormone (GHRH) is limited by its short half-life. Also it follows that Sermorelin faced the same difficulties and thus is limited by its short half life (approximately 12 min following intravenous injection in humans), mainly due to its susceptibility to rapid enzymatic degradation. Thus, the product quickly dissipated in the body and the peptide could not stay in the body long enough to have a medicinal impact. A Munafo, T X Q Nguyen, O Papasouliotis, H L?cuelle, A Priestley and M O Thorner (2005). There were attempts to resolve some of the short half-life issues with Sermorelin, in fact a PEGylated GHRH was developed. Even though PEGylated GHRH solved some of the degradation issues, the much more potent CJC 1295, rendered PEGylated GHRH obsolete.

Instead of using a PEGylated technology, the technology of bioconjugation was employed. In vivo bioconjugation to serum albumin is a useful tool to increase the half-life of small molecules or peptides in plasma. In vivo bioconjugation occurs when a strategically placed reactive group on a bioactive peptide reacts with a nucleophilic entity found in blood or in sc interstitium to form a stable bond. The foremost nucleophile is the thiol, and its most abundant source in these fluids is Cys34 on albumin. The thiol on Cys34 reacts with a Michael acceptor, such as a maleimido derivative, leading to a new bioactive protein construct that will adopt an extended half-life due to stabilization from enzymatic degradation) or reduced elimination through the kidney. It therefore became logical to combine the long-lasting effect of bioconjugation with the proper GRF analog. Lucie Jett?, Roger L?ger, Karen Thibaudeau, Corinne Benquet, Martin Robitaille, Isabelle Pellerin, V?ronique Paradis, Pieter van Wyk, Khan Pham and Dominique P. Bridon (2005).

CJC-1295 is a synthetic modification of growth hormone releasing factor (GRF) with D-Ala, Gln, Ala, and Leu substitutions at positions 2, 8, 15, and 27 respectively. These substitutions create a much more stable peptide with the substitution at position 2 to prevent DPP-IV cleavage, position 8 to reduce asparagine rearrangement or amide hydrolysis to aspartic acid, position 15 to enhance bioactivity, and position 27 to prevent methionine oxidation. By applying the Drug Affinity Complex (DAC) technology to GRF, the peptide selectively and covalently binds to circulating albumin after subcutaneous (SC) administration, thus prolonging its half-life. These substitutions are key in increasing the overall half life of CJC-1295 but there lies an even greater reason as to why the half life has been extended from ~7 minutes to greater than 7 days. Bioconjugation takes a reactive group and attaches it to a peptide, which in turn reacts with a nucleophilic (usually a partially negative molecule) entity found in the blood to form a more stable bond. Albumin, one of the most abundant substances in the human body is chosen as the nucelophile by this particular peptide thanks to a Cys34 thiol group that attracts it. By combining the tetrasubstituted GHRH analogue with maleimodoproprionic acid using a Lys linker, you create a GHRH peptide with a high binding affinity for albumin.

So how effective is bioconjugation? How long will CJC-1295 stay in ones system? How will CJC-1295 impact IGF-1 levels? This is the exact question researchers asked and a study was conducted to determine the efficacy of CJC-1295. The objective of this study was to examine the pharmacokinetic profile, pharmacodynamic effects, and safety of CJC-1295, a long-acting GHRH analog. The study design was two randomized, placebo-controlled, double-blind, ascending dose trials with durations of 28 and 49 days. Healthy subjects, ages 21-61 years old were studied. After a single injection of CJC-1295, there were dose-dependent increases in mean plasma GH concentrations by 2- to 10-fold for 6 days or more and in mean plasma IGF-I concentrations by 1.5- to 3-fold for 9-11 days. The estimated half-life of CJC-1295 was 5.8-8.1 days. After multiple CJC-1295 doses, mean IGF-I levels remained above baseline for up to 28 days. No serious adverse reactions were reported. Sam L. Teichman, Ann Neale, Betty Lawrence, Catherine Gagnon, Jean-Paul Castaigne and Lawrence A. Frohman (2006). What was the research dose used in the study? A particularly important question, the dosage was 30-60 micrograms per kilogram of bodyweight.

This bears repetition, GH remained elevated for up to six days! IGF-1 concentrations were up 1.5 to 3 fold for 9-11 days! And the estimated half-life of CJC-1295 is 5.8-8.1 days! IGF-1 levels were elevated up to 28 days! At a dosage of 30-60 micrograms per kilogram of bodyweight, with no significant side effects. Excuse all he emphasis but this is a truly remarkable research product, its ability for efficacy is self-evident.

So in sum, what is CJC-1295? CJC-1295 is a long-acting analog of GH-releasing hormone. CJC-1295 exhibits the same effects of Human Growth Hormone, it has the ability to promote muscle mass, increase bone density, improve protein synthesis, increase IGF-1 levels potently, strengthen immune systems, stimulate the production of bone marrow cells that produce red blood cells, and of course reduce excess body fat, especially abdominal fat. (The reduction of abdominal fat is the single most profound effect of HGH replacement.)

Peptide should be administered at least twice a week (so divide the research dose into two administrations on your research subject) this will help to keep blood levels consistent in your research subject, or in cellular culture, or in vitro.

Building Muscle with Growth Hormone Releasing Peptides

Growth hormone can be a very confusing subject to those who don't take the short time it takes to understand how it works in our body, how it is released in our body, and how we as people can manipulate these secretions.

For those that do some research and learn how to use these powerful compounds, the potential to create new muscle is unlimited. Peptides are here to become the next generation of performance enhancement and muscle building compounds. Taking a short time to  gain an understanding about how they work, will be the key to gaining huge amounts of muscle and untold brute strength, while burning bodyfat permanently. First, lets take a look at out bodies normal GH, how it is released, and the benefits it has on us.

Growth Hormone is a 191-amino acid polypeptide that is produced naturally in our body by our anterior pituitary. HGH is a protein that stimulates the body cells to increase both in size, as well as undergo more rapid cell division than usual. It enhances the movement of amino acids through cell membranes and also increases the rate at which these cells convert these molecules into proteins. It also increases the body's IGF levels, which is most likely the sole reason for reported muscle gains in users, and IGF is well known for muscle and cartilage repair. This growth hormone, called Somatotropin, is different then the normal HGH that we think of when we think of bodybuilder and athletes using GH for performance enhancement. HGH is really rHGH, or "recombinant HGH" which means it was made using DNA technology. This rHGH is called Somatropin, and is what is found being sold on the black market to bodybuilders and athletes. Now here's the sad part, almost all of the black market rHGH today is not the 191 amino rHGH that you are expecting to put into your body. True, 191 amino HGH is 22kda in weight, and I can almost guarantee you wont find that in the fridge of any of your local gym rats. Companies have removed aminos from the chain, as 191 aminos is a very long and difficult chain to produce. Many producers have found that folding the amino chain over on top of itself to shorten it work to their benefit. Either way, when you inject rHGH into your body, you are introducing a sub-part substitute to your natural GH and may eventually effect or inhibit your natural producing of that quality GH.

GROWTH HORMONE RELEASING HEXAPEPTIDE
So what can be done about this? This is where the new peptides come in, GHRP and GHRH. First we will look at GHRP(6). GHRP, or Growth Hormone Releasing Hexapeptide, is a GH secretagogues peptide. What does that mean? Well, when introduced into our body, GHRP induces our pituitary to secrete GH. What is more interesting is that it does it from a completely different receptor then GHRH does (we will get to GHRH later). These GHRP secretagogues act like synthetic ghrelinmimetics (keyword being mimetics). This is the reason for hunger reported with GHRP use, because ghrelin is naturally released by the stomach lining to induce hunger in humans. Because GHRP stimulates our natural secretion from the pituitary, we are getting the top quality, 191 amino GH in our bodies... no gimmicks and no substitutes. We are getting elevated levels of this, and the best part is it is being produced by our own body. rHGH use has been known to inhibit natural production, and GHRP has been used to restart a persons natural production. Other peptides that fall into the GHRP category are hexarelin, and Ipamorelin.

GROWTH HORMONE RELEASING HORMONE
On the other side, saturating a totally different receptor, we have GHRH. A growth hormone releasing hormone is a 44 amino peptide that is produced and our hypothalamus, and sent down to our pituitary to stimulate the growth hormone releasing hormone receptor (GHRH-r) as a signal to start producing GH. An added benefit of GHRH is that it promotes slow-wave sleep directly, and can stimulate the pituitary alongside of a GHRP. An example of a GHRH would be CJC-195. Besides the advantage of our own natural GH, another advantage of CJC is that it has a much more prolonged effect on our GH levels in the body (increased half-life).

DOSING
So now, we move onto dosing. We will start with  - GHRP-6. I like using GHRP-6 post workout, before my meal. It helps me increase my nutrition uptake, and it is also great at that time because I pin IGF pre workout (which is on the negative feedback loop), so pinning GHRP later will keep your body up and producing GH naturally and working with your natural IGF. Also, mid day is when your GH levels are at their lowest, so this is an optimal time to pulse. Dosing ranges from the minimal 100mcg, to what I take, 500mcg. For a GHRH, we want to pretty much base it off the same time schedule, but days taken will be a little different. For CJC1295 DAC, we want to dose it from 1mg to 2mg per week, splitting the dose up in half and taking them 3 days apart. For example, 1mg on Monday and 1mg on Thursday is a fine and effective protocol. As for all the others, GHRP-2, hexarelin, Ipamorelin, CJC no-DAC well you're capable of researching them on your own. For even better results, we can pulse GHRP while we have CJC in our systems, hitting our pituitary from both types of receptors, and keeping it doing its natural GH pulse. For this reason supplementing a GHRP with a GHRH yields great results.

How To Get The Most Natural HGH Secretion For Your Genetics

One of the best natural ways to get the most natural HGH secretions for your genetics is to workout at a high intensity for periods no longer than 45 minutes.
This is according to the Bulgarians Olympic power lifting studies. I personally believe you should be monitoring your affected muscles for the amount of blood
gorging they have.

The higher your testosterone and growth hormone levels, considering your diet and rest is optimal as well, you will be able to maintain a pump for longer. You
will also have a fuller pump! Cardiovascular health is crucial to saturate the blood capillaries with oxygen. Start with the goal of finishing your workouts around the forty five minute mark. If you feel that you lose your pump before this time is up, then before you completely lose your pump, quit to keep as much of the pump for as long as possible.
On the other hand if you still have a good pump at the end of the forty five minute period I suggest you keep pushing yourself until you feel your pump starting to subside. This way you can get the most amount of growth without overtraining in the least amount of time possible.Pushing yourself after your pump has subsided is a double edged sword cutting your progress in half!